OLIGOSYNTHESIS
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Oligosynthesis
Oligo Synthesis
Bioserve India, A part of the Reprocell group of companies is the pioneer for Oligonucleotide synthesis in India. Since its inception, Bioserve delivers the highest quality products to match the researcher’s needs. We offer a complete line of custom Oligonucleotide synthesis and purification services based on customer requirements. We facilitate the synthesis of Oligonucleotides with the help of High-throughput automated machines.
The ancillary reagents used for the synthesis are tested and approved before use in production to avoid low-quality parameters. The synthesis process is optimized and automated to meet the stringent requirements of quality level. Moreover, we work according to GMP procedures, providing full traceability with extensive batch production records.
We provide exceptional quality Oligonucleotides which enables you to succeed in your experiments
- Oligos are synthesized on 10, 20, 25, 50, 100, 200 and 1000 nmole scales.
- Other quantities available on request. All the oligos are desalted & lyophilized.
- Increase your quality based on your experimental needs. We offer wide range of purification techniques.
- We follow stringent synthesis and QC parameters to provide quality product to our customers.
Choose the right Scale and Purification
| Type of Purification | Length | 5-35 | 5-35 | 5-50 | 5-100 | 5-120 | 5-150 |
|---|---|---|---|---|---|---|---|
|
|
Scale (nmole) |
10 |
20 |
50 |
100 |
200 |
1000 |
|
Desalted |
OD @ 260 nm |
5-7 |
7-8 |
8-10 |
15-20 |
21-25 |
30-35 |
|
OPC |
OD @ 260 nm |
1-3 |
2-4 |
2-5 |
5-8 |
8-12 |
12-15 |
|
HPLC |
OD @ 260 nm |
- |
- |
1-4 |
2-5 |
8-10 |
10-12 |
|
PAGE |
OD @ 260 nm |
- |
- |
1-3 |
3-5 |
5-7 |
7-10 |
Standard Modifications
| Modifications | Labelling Pos. | - | - |
|---|---|---|---|
|
|
5' |
Int |
3' |
|
BIOTIN |
✓ |
- |
✓ |
|
INOSINE |
✓ |
✓ |
- |
|
AMINO |
✓ |
- |
✓ |
|
PHOSPHORYLATION |
✓ |
- |
✓ |
|
THIOL |
✓ |
- |
✓ |
Quenchers
| 5´ Label | 3’ Label |
|---|---|
|
FAM |
BHQ-1 |
|
TET |
BHQ-1 |
|
HEX |
BHQ-1 |
|
TAMRA |
BHQ-1, BHQ-2 |
|
CY5 |
BHQ-2 |
|
CY3 |
BHQ-1 |
Details of Activity Report
- OD Units
- Oligo concentrations in picomoles, nanomoles and micrograms
- Melting Temperature (Tm) of the Oligo
- Total Yield
- GC Percentage
- Molecular Weight
- Reconstitution volume to get the 100µM concentration
Long, high-quality Oligos are needed by demanding applications such as cloning, ddRNAi, homology-directed repair, and gene construction.
We generate Long Mer DNA Oligonucleotides using proprietary synthesis methods which deliver high-quality Oligos up to 150 bases. They are available singly or as double-stranded and can be delivered in tubes or plates.
Enjoy complete confidence in Oligos that are verified by electrospray ionization-mass spectrometry.
- Fluorescent Labelled
For fluorescent detection of target amplification, there is a range of primer designs with different primer and probe-based detection chemistry. Fluorescence quenching is used for most fluorescent probes, in which a fluorescent reporter is quenched by a quenchers proximity before the primer hybridises to a single nucleic acid strand. We provide multiple fluorescent groups and quenchers at 5′ or 3′ designated or customer specified sites are also given. FAM, HEX, TET, TAMRA, and many more are included in this for several applications, including PCR, cloning, sequence verification and gene detection.
Available dual-labelled modifications with quenchers
- Non-Fluorescent Labelled
Modern sequencing uses the same basic technology as PCR, with extension followed by the binding of a primer to single-stranded DNA, while various platforms use various technologies to read the resulting sequence of bases. Non-Fluorescent Labelled Oligonucleotides do not compromise of any fluorescence molecules in neither of the ends, but it might have internal modifications like Biotin, Inosine, and an end to end modifications like Phosphorylation, Amino and other related modifications based on the applications.
Reduce barcode crosstalk in your multiplexed NGS experiments
Quality requirements for synthetic Oligos used in Next-generation sequencing (NGS), such as indexed adapters and barcoded fusion primers, have reached unprecedented high levels. Traditional purification methods (e.g., HPLC) can effectively increase the number of full-length Oligos but are incapable of reducing cross-contamination that can occur in co-synthesized Oligos. In contrast to less sensitive applications where such Oligo-to-Oligo crosstalk can go unnoticed, the use of indexed adapters containing even low levels of cross-contamination can lead to barcode misalignment and sample misidentification, which may confound experiments requiring detection of rare variants. NGS Grade Oligos are manufactured by proprietary methods that are proven to reduce index cross-talk and increase the success of multiplex NGS experiments. With NGS Grade oligos, you can:
- Obtain DNA oligos with cross-contamination levels as low as 0.01%
- Select from a variety of product options to suit your requirements
- Experience the industry-leading quality and turnaround time for which BioServe is known.
Oligo Applications:
Our oligos come with a guarantee of high performance for a wide spectrum of research applications including:
- Sequencing
- Microsatellite Genotyping
- PCR and RT-PCR
- Cloning
- RAPD and Universal Primers
- Wobble bases
- Diagnostics
Bioserve India provides the following methods for Purifying Oligonucleotides
Desalted:
All oligos synthesized are processed through normal phase chromatography column which removes salts but not failure sequences. Desalted oligos are ready-to-use, suitable for many PCR and sequencing applications without further purification.
Recommended Applications Like PCR, AFLP, RFLP
OPC:
This purification method is based on the “trityl-on” synthesis of the oligonucleotides. Purification on reverse phase cartridge ensures higher purity scales. It removes failure sequences. As the length of oligos increases, the proportion of truncated sequences tends to increase. These impurities will not be removed by Cartridge and thus for longer oligos, HPLC or PAGE is recommended.
Recommended Applications: Like Sequencing, RFLP, DNA Fingerprinting, RT-PCR, First strand DNA synthesis. Cartridge purification is the recommended purification for Oligos greater than 35 to 50 bases.
HPLC:
BioServe India provides both reverse phase and ion exchange HPLC purification of oligonucleotides. HPLC is an efficient purification method for oligos with fluorophores, as their intrinsic lipophilicity provides excellent separation of product from its contaminants. RP-HPLC is a method of choice for larger scales due to the capacity and resolving properties of the column. As resolution depends on lipophilicity it will decrease with the length of the Oligo. RP-HPLC is usually not recommended for purifying products longer than 60 bases.
Recommended Applications like Antisense studies, Cloning adapters, Real Time PCR.
PAGE:
In this procedure, oligonucleotides are purified based on their mobility on denaturing polyacrylamide gels. The band of interest is excised using UV shadowing and extracted from the gel. Oligonucleotides purified by this method are 99% full length. PAGE is the recommended purification for longer oligos (>50 bases).
Recommended Applications like Mutagenesis, Gene Synthesis, Primer Extension, Cloning adapters.
Committed Guaranteed yield on synthesis parameters
OD:
All the oligonucleotides synthesized at BioServe-India are quantitated using the absorbance at A260.
Gel Analysis:
All the oligonucleotides synthesized are electrophoresed on a polyacrylamide gel to make sure that they are of the requested length and there are no n-1 oligonucleotides. This gel picture will be provided to the customer.
Each shipment includes data sheets for import into customers’ information systems containing well location, sequence, oligo name, lot number, MW, µg/OD, length, Tm, concentration and volume (in solution)
Universal Primer
Universal primers are complementary to nucleotide sequences used for the amplification of a very similar gene that related to a specific Genus. It binds to a wide variety of DNA templates.
