Modified DNA Oligos
  • Fluorescent Labelled

For fluorescent detection of target amplification, there is a range of primer designs with different primer and probe-based detection chemistry. Fluorescence quenching is used for most fluorescent probes, in which a fluorescent reporter is quenched by a quenchers proximity before the primer hybridises to a single nucleic acid strand. We provide multiple fluorescent groups and quenchers at 5′ or 3′ designated or customer specified sites are also given. FAM, HEX, TET, TAMRA, and many more are included in this for several applications, including PCR, cloning, sequence verification and gene detection.

 

Fluorescent Dye Abs Em Ext. Coeff. MW Labelling Pos. Purification Alternative to
5′ Int 3′ HPLC
Fluorescein [FLU] 495 520 83 504,00     x x
FAM [FAM] 495 520 83 474,50 x     x ATTO 495
HEX [HEX] 535 556 73 681,20 x     x CAL Fluor 560 SIMA
TET [TET] 521 536 73 612,30 x     x CAL Fluor Gold 540
TAMRA [TAM] 544 576 90 512.58 / 612.70 x   x x
Cyanine3 [CY3] 552 570 150 444,60 x     x TYE 563
Cyanine5 [CY5] 649 670 250 470,63 x     x TYE 655

 

Available dual-labelled modifications with quenchers

 

Dyes Quenchers Purification
5’6-FAM 3’TAMRA HPLC/PAGE
3’BHQ-1
3’BHQ-2
5’TET 3’TAMRA
3’BHQ-1
3’BHQ-2
5’HEX 3’TAMRA
3’BHQ-1
3’BHQ-2
5’TAMRA 3’BHQ-1
3’BHQ-2
5’CY3 3’BHQ-2
5’CY5 3’BHQ-1
3’BHQ-2

 

  • Non-Fluorescent Labelled

Modern sequencing uses the same basic technology as PCR, with extension followed by the binding of a primer to single-stranded DNA, while various platforms use various technologies to read the resulting sequence of bases. Non-Fluorescent Labelled Oligonucleotides do not compromise of any fluorescence molecules in neither of the ends, but it might have internal modifications like Biotin, Inosine, and an end to end modifications like Phosphorylation, Amino and other related modifications based on the applications.

 

Modification 5′ Int 3′ Purification Option
DESALTING HPLC PAGE
Amino X x x x x
Phosphorylation X x x x x
Thiol X x x x
Biotin x x x x x
Inosine x x x x x x

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