Universal Primer

Universal primers are complementary to nucleotide sequences used for the amplification of a very similar gene that related to a specific Genus. It binds to a wide variety of DNA templates.

 

S.No Primer Name Primer Sequence
1 M13 Reverse (-27) 5′-GGA AAC AGC TAT GAC CAT G-3′
2 M13 Forward (-41) 5′-GGT TTT CCC AGTC ACG AC-3′
3 M13 Forward (-20) 5′-GTA AAA CGA CGG CCA GTG-3′
4 M13 Forward (-21) 5′-TGT AAA ACG ACG GCC AGT-3′
5 M13 Reverse (-48) 5′-AGC GGA TAA CAA TTTC ACA C-3′
6 SP6 5′-TAC GAT TTA GGT GAC ACT ATA G-3′
7 T3 5′-CAA TTA ACC CTC ACT AAA GG-3′
8 T7 5′-TAA TAC GAC TCA CTA TAG GG-3′
9 T7 EEV 5′-ATGTCGTAATAACCCCGCCCCG-3′
10 T7 Reverse 5′-TAGTTATTGCTCAGCGGTGG-3′
11 T7 Term 5′-GCT AGT TAT TGC TCA GCG G-3′
12 pBluescript KS 5′-TCG AGG TCG ACG GTA TC-3′
13 pBluescript SK 5′-CGC TCT AGA ACT AGT GGA TC-3′
14 3’pGEX 5′-CCG GGA GCT GCA TGT GTC AGA GG-3′
15 5’pGEX 5′-GGG CTG GCA AGC CAC GTT TGG TG-3′
16 GST-Tag 5′-ACC CAA TGT GCC TGG ATG CG-3′
17 pTrcHis-Forward 5′-GAG GTA TAT ATT AAT GTA TCG-3′
18 pTrcHis-Reverse 5′-GAT TTA ATC TGT ATC AGG
19 CMV-Forward 5′-CGC AAA TGG GCG GTA GGC GTG-3′
20 CMV-Reverse 5′-AGT AGG AAA GTC CCG TAA GG-3′
21 EGFP-C 5′-CAT GGT CCT GCT GGA GTT CGT G-3′
22 EGFP-N 5′-CGT CGC CGT CCA GCT CGA CCA-3′
23 BGH-Reverse 5′-TAG AAG GCA CAG TCG AGG-3′
24 pQEproseq 5′-CCC GAA AAG TGC CAC CTG-3′
25 pQErevseq 5′-GTT CTG AGG TCA TTA CTG G-3′
26 Intein Forward 5′-CCC GCC GCT GCT TTT GCA CGT GAG-3′
27 5′-pBabe-Seq 5′-CTT TAT CCA GCC CTC AC-3′
28 3′-pBabe-Seq 5′-ACC CTA ACT GAC ACA CATT CC-3′
29 -96 glll Sequencing Primer 5′-CCC TCA TAG TTA GCG TAA CG-3′
30 GAL1 Forward 5′-AAT ATA CCT CTA TAC TTT AAC GTC-3′
31 pBAD Forward 5′-ATG CCA TAG CAT TTT TAT CC-3′
32 pBAD Reverse 5′-GAT TTA ATC TGT ATC AGG
33 pTRE 3′ 5′-CCA CAC CTC CCC CTG AAC-3′
34 pTRE 5′ 5′-CGC CTG GAG ACG CCA TCC-3′
35 pYESTrp Forward 5′-GAT GTT AAC GAT ACC AGC C-3′
36 pYESTrp Reverse 5′-GCG TGA ATG TAA GCG TGA C-3′
37 RVprimer3 5′-CTA GCA AAA TAG GCT GTC CC-3′
38 Rvprimer4 5′-GAC GAT AGT CAT GCC CCG CG-3′
39 GLprimer 1 5′-TGT ATC TTA TGG TAC TGT AAC TG-3′
40 GLprimer 2 5′-CTT TAT GTT TTT GGC GTC TTC CA-3′
41 SeqL-A (ATTL1) 5′-GCG AGA GTA GGG AAC TGC-3′
42 SeqL-B (ATTL2) 5′-AAC ATC AGA GAT TTT GAG ACA C-3′
43 SV40-pArev 5′-CCT CTA CAA ATG TGG TAT GG-3′
44 SV40-Promoter 5′-GCC CCT AAC TCC GCC CAT CC-3′
45 U6 Primer 5′-GGG CAG GAA GAG GGC CTA T-3′
46 Xpress Forward 5′-TAT GGC TAG CAT GAC TGG T-3′
47 EBV-Rev primer 5′-GTG GTT TGT CCA AAC TCA TC-3′
48 hU6-01 5′-GAG GGC CTA TTT CCC ATG ATT-3′
49 hU6-02 5′-TAA TTA GAA TTA ATT TGA CT-3′
57 BGHR 5′-TAGAAGGCACAGTCGAGG-3′
58 CMVF 5′-CGCAAATGGGCGGTAGGCGTG-3′
59 PGEXFP 5′-GGGCTGGCAAGCCACGTTTTGGTG-3′
60 PGEXRP 5′-CCGGGAGCTGCATGTGTCAGAGG-3′
61 T3 5′-ATTAACCCTCACTAAAG-3′
62 SP6 5′-AGCTATTTAGGTGACACTATAG- 3′
63 ITS1 5′-TCCGTAGGTGAACCTGCGG-3′
64 ITS4 5′-TCCTCCGCTTATTGATATGC-3′
65 ITS2 5′-GCTGCGTTCTTCATCGATGC-3′
66 ITS3 5′-GCATCGATGAAGAACGCAGC-3′
67 27F/16SF 5′-AGAGTTTGATCMTGGCTCAG-3′
68 1492R/16SR 5′-TACGGYTACCTTGTTACGACTT-3′
69 1525R 5′-AAGGAGGTGWTCCARCC-3′
70 pGEX3 5′-GGAGCTGCATGTGTCAGAGG-3′
71 pGEX5 5′-GGCAAGCCACGTTTGGTG-3′
72 RV3 5′-CTAGCAAAATAGGCTGTCCC-3′
73 pJET1.2F 5′-CGACTCACTATAGGGAGAGCGGC-3′
74 pJET1.2R 5′-AAGAACATCGATTTTCCATGGCAG-3′
75 SV40-Promoter 5′-GCCCCTAACTCCGCCCATCC-3′
76 EGFP-N 5′-CGTCGCCGTCCAGCTCGACCAG-3′
77 EGFP-C 5′-CATGGTCCTGCTGGAGTTCGTG-3′
78 VP1.5F 5′-GGACTTTCCAAAATGTCG-3′
79 XL39R 5′-ATTAGGACAAGGCTGGTGGG-3′
80 T5 Promoter 5′-GTG AGC GGA TAA CAA TTT CAC AC-3′

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