What Are Off-Target Effects in Cellular Gene Editing? Risks, Detection, and Solutions
What Are Off-target Effects in Gene Editing? Off-target effects in gene editing refer to unintended genetic modifications occurring at genomic loci other than the intended target site. In CRISPR-based gene editing systems, these arise because the guide RNA (gRNA), which directs the system to the target DNA sequence, can also bind to DNA sequences with…