CLINICAL DIAGNOSTICS
Colorectal Cancer Multigene Panel
Colorectal Cancer Multigene Panel (CRC CARE)
Colorectal cancer (CRC) is a formidable health problem worldwide and fourth most common cause of death due to cancer. In India, the annual incidence rates (AARs) for colon cancer and rectal cancer in men are 4.4 and 4.1 per 100000, respectively.
Causes
An estimated 10% -15% of these cancers are likely attributable to hereditary (germline) causes. Several genes are associated with an increased risk of developing CRC, and those of key interest include those for Lynch syndrome, MLH1, MSH2, MSH6, PMS2, EPCAM; adenomatous polyposis conditions (APC), MUTYH, POLE, POLD1; hamartomatous polyposis syndromes PTEN, SMAD4, STK11, and other rare cancer predisposition states where colorectal cancer is part of the phenotype, CHEK2 and TP53.
The National Comprehensive Cancer Network™ (NCCN) guidelines provide recommendations for investigating MSI by PCR and/or MMR by IHC for 15 different cancer types
Other environmental factors include: Age, gender (male), ulcerative colitis, alcohol, obesity etc.
Genes Covered
APC BRAF CDKN2A ERBB2 KRAS MLH1 MSH2 MSH6 NRAS PIK3CA PMS2 POLE PTEN SMAD4 TP53 MUTYH STK11 EPCAM BMPR1A CHEK2 POLD1
Why Genetic Testing
- The latest NCCN guidelines recommend genetic evaluation if younger than age 50 regardless of other test results/ family history.
- The germline MGPT (multigene panel test) strategy is an alternative to tumor and family history-driven selection of patients with CRC for genetic testing because it is more sensitive for identifying individuals with LS and other cancer risk genes than a strategy of selecting for germline testing based on family history and tumor-based criteria.
- Pathogenic variants identified by MGPT are clinically actionable and inform screening and surveillance recommendations the treatment implications for patients with CRC and pathogenic mutations in the Lynch syndrome MMR genes are the best characterized and include response to immune checkpoint inhibitor therapy/programmed death-1 (PD-1) inhibitors.Keytruda (pembrolizumab) is approved for the treatment of any solid tumours that test MSI-H, have progressed after treatment and for which there are no other treatment options.
Specimen Requirements
- 3 ml BLOOD in EDTA/ lavender top tubes
- FFPE blocks
Our Other Panels!
We offer several cancer panels depending upon the needs of the Oncologists, these include..
BRCA 1/2 Gene Testing
BRCA panel for testing mutations in BRCA1 and BRCA2 genes
Lung RNA Fusion Panel
Lung RNA fusion panel for detecting gene fusions in major driver genes
BRCA Extended Panel
Profiling of 15 genes associated with hereditary Breast and Ovarian cancer, pancreatic and prostate cancer
Hereditary Cancers panel
Profiling of >140 genes associated with inherited cancers