All Services / Sanger Sequencing

Automated DNA sequencing is normally one of the most common and robust techniques performed. Bioserve’s DNA sequencing service has been designed for rapid delivery and great value. Our valued customers can download sequence data within a week of their samples being received. With over 15 years of experience in Sanger sequencing, we employ streamlined technology platforms for sequencing with the latest state-of-the-art ABI 3730 48-capillary DNA Analysers along with our proprietary reagents for optimized and highly accurate performance. Our dedicated team of professionally trained experienced scientists use state-of-the-art instruments to provide an exceptionally reliable service that meets all your DNA sequencing needs. You can rely on us to produce high-quality results in the best possible time. Select your free primers from our list of available universal primers, use our oligonucleotide synthesis service for unique primer design or send your primers along with your samples.

  • Culture Sequencing
  • Fast-track seq/Gen-scan
  • Ready to Run/Plate
  • Miscrosatellite Genotyping
  • Plasmid/PCR sequencing
  • Primer Walking
  • Package (Extraction to Data Analysis)
Service includes isolation of plasmid from culture and then sequencing
 
Rapid turn around time

4-5 working days

Sample type

Bacterial culture

 
Flexible sample submission

Accept culture plates, stabs, slants

Report format

Raw Chromatogram File (.abi file) Upon request we provide FASTA formats and PDF formats to customers.

 
Catalogue No.

SS-CS

This service is offered to all customers at an extra charge.

 
Rapid turn around time

Sequencing results are delivered within 24hrs from receipt of samples.

 
Sample type

Sequencing samples can be sent as clones, unpurified PCR products and PCR purified products or Genescan PCR produtcs

 
Flexible sample submission

Accept samples submitted in different tube formats: 1.5ml tubes, strip tubes and 96-well plates

 
Sample concentration and volume

Plasmid- 100ng-500ng/ul.PCR- 40ng-100ng/ul. Volume should be minimum 10ul for two reactions.

 
Primer volume/concentration

Primer volume should be minimum of 10ul with 5pm/10pm concentration

This service is offered for pre-cycled sequencing reactions in single tubes or in plates.

 
Rapid turn around time

1 working day/Plate

Sample Type

96-well sequencing/genscan sample plate resuspended in formamide which are directly to be loaded into machine

Flexible sample submission

Samples should be sent in 96-well plates or PCR tubes properly labelled and sealed.

Report Format

Raw Chromatogram File(.abi file)

Catalogue-No

SS-RTR-S Ready to run Sequencing.SS-RTR-GS Ready to run Genescan plates

Miscrosatellite Genotyping

Short Tandem Repeats (STRs) are also called Microsatellites. These are polymorphic DNA loci present throughout the genome and used for linkage mapping studies, association studies, and identification of organisms. Microsatellite Instability (MSI) is a form of genomic instability arising from reduced fidelity during the replication of repetitive DNA that often occurs in tumor cells. Microsatellite ISSR  – Inter-simple sequence repeat (ISSR) analysis is a technique commonly used for identifying individuals, commonly in plant species.

Our single-read services enable routine sequencing of plasmids, PCR products.

 
Rapid turn around time

Sequencing results are delivered within 3-4 working days from receipt of your samples.

Sample Type

Samples can be sent as clones, un-purified PCR products and PCR purified products

Flexible sample submission

Accept samples submitted in different tube formats: 1.5ml tubes, strip tubes and 96-well plates

Sample Concentration and Volume

Plasmid- 100ng-500ng/ul.PCR- 40ng-100ng/ul. Volume should be minimum 10ul for two reactions.

Primer Volume/Concentration

Primer volume should be minimum of 10ul with 5pm/10pm concenatrtion

Report Format

Raw Chromatogram File(.abi file) Upon request we provide FASTA formats and PDF formats to customers.

Primer walking is a sequencing method of choice for sequencing DNA fragments between 1.3 and 7 kilobases. Such fragments are too long to be sequenced in a single sequence read using the chain termination method. This method works by dividing the long sequence into several consecutive short ones.

 
Rapid turn around time

Primer walking is the consecutive design of new primers to extend into unknown sequence. It takes ~4 days for each primer "step". The longer you need to "walk", obviously the longer this process will take. For just a few kb, this is the best method.

Sample type

Plasmid DNA sample

Flexible sample submission

Accept samples in 1.5ml tubes

Sample concentration and volume

100ng-500ng/ul. Volume should be around 20ul.

Report format

Raw Chromatogram File(.abi file) Upon request we provide FASTA formats and PDF formatsto customers.

Catalogue No.

SS-PW

This service includes genomic DNA extraction, amplification, and sequencing.

 
Rapid turn around time

Upon request

Sample type

Accept blood samples, cultures (bacterial/fungal)

Flexible sample submission

Accept culture plates, stabs, Slants. 5ml. Blood samples in EDTA coated vials, culture plates, stabs.

Report format

Raw Chromatogram File (.abi file) Upon request we provide FASTA formats and PDF formats to customers.

Catalogue No.

SS-WS