Sanger Sequencing Services

At Bioserve, we provide high-quality Sanger sequencing services for PCR products, plasmids, and other DNA samples with fast turnaround times and exceptional accuracy. With over 15 years of expertise, our advanced ABI 3730 48-capillary DNA analyzers and optimized sequencing protocols ensure precise, high-resolution results for your research, diagnostics, or cloning projects.

What is Sanger Sequencing?

Sanger sequencing is the gold-standard method for accurate DNA sequencing, ideal for validating genes, detecting mutations, and confirming PCR/plasmid constructs. It uses fluorescent- labelled terminators and capillary electrophoresis to deliver read lengths up to 700+ bases with >99.9% accuracy.

Why Choose Bioserve for Sanger Sequencing?

✅ Fast & Efficient Service – Receive your sequencing data within 3-4 business days after sample submission.
✅ High Accuracy & Read Lengths – Our proprietary reagents and advanced technology deliver up to 700+ base pairs of high-quality sequence data.
✅ Expert Team – Our experienced scientists ensure reliable, reproducible results for every sample.
✅ Flexible Primer Options – Use our free universal primers, order custom primers, or supply your own.
✅ Competitive Pricing – Affordable sequencing services without compromising quality.

Our Services

1. PCR Product Sequencing

Confirm the accuracy of your PCR-amplified DNA fragments with our high-fidelity sequencing. Ideal for:

Mutation detection
Genotyping
Cloning verification

2. Plasmid DNA Sequencing

Ensure your cloned inserts, promoter regions, or gene constructs are error-free with our plasmid sequencing service. We support:

Insert confirmation
SNP analysis
Primer walking for long fragments
Gene edits/knockout confirmation

3. Custom Primer Sequencing

Need a specific primer for your project? Our oligonucleotide synthesis service provides high-quality primers tailored to your needs. We can design as well as synthesize primer for your sequencing needs.

4. Molecular Identification services:

We offer reliable molecular identification of species, strains, or clones through sanger sequencing. Our service provides high-quality, sequence data for confirming genetic identity in research, clinical, and industrial applications. We can handle all types of sample types (microbes/plants/animal tissues) under DNA Barcoding services.

How It Works – Simple & Hassle-Free

Prepare Your Sample – Submit PCR products, plasmids, or purified DNA (min. 50 ng/µl).
Choose Your Primers – Select from our universal primers or provide your own.
Submit Your Samples – Ship your samples to our lab or use our online submission portal.
Get Your Results – Download high-quality chromatogram files (ab1) and sequence data within days.

Sample Submission Guidelines

Sample Type	Concentration	Volume	Purity (A260/280)
PCR Products	≥ 0 ng/µl	10 µl	1.7 – 2.0
Plasmid DNA	≥100 ng/µl	10µl	1.8 – 2.0
Genomic DNA	≥ 100 ng/µl	10 µl	1.7 – 2.0

Note: For difficult templates (high GC content, repeats, or secondary structures), please contact us for optimization.

Trusted Sequencing Partner for Researchers Worldwide

Academic labs, biotech firms, and pharmaceutical leaders rely on Bioserve’s Sanger sequencing services for accurate, cost-effective DNA analysis. From clone verification to mutation screening and PCR product analysis, we deliver precision results with fast turnaround times through cutting-edge technology and expert support. Experience high-confidence sequencing data tailored to your research needs.

Get a Free Quote: Fill out our contact form below, and our sequencing team will reach out to you within 24 hours.

Frequently Asked Questions

What’s the cost per sample?
Prices start at — with discounts for bulk orders. Request a custom quote.
How soon will I get results?
Typically 2-3 business days after we receive your samples.
Do you analyze the data?
We provide raw sequence files. Optional analysis services are available.
Can you sequence difficult samples?
Yes! For GC-rich, repetitive, or complex templates, we follow a separate protocol (pricing may vary).
What about primers?

We have many options:

Send us your primers along with the sample
let us know the sequence, we can synthesisze it for you
Let us know your region of interest, we can design and synthesise the primers for you

If your sequencing requires usage of Universal primers ( T7/M13) check with our team, we may already have them with us.

Sanger Sequencing Services