Qkine Growth Factors and Cytokines
See also:
QK052
FGF2-G3 (145 aa) protein is a thermostable engineered form of FGF-2 (bFGF). Qk052 comprises the 145 aa form of FGF-2 (Qk025) with the nine amino acid substitutions developed by Dvorak et al. 2018. This increases the functional half-life of the protein from <10 h (wild-type) to >7 days (FGF2-G3 145 aa).
FGF2-G3 is used in B8 media (Kuo et al. 2019) for weekend free, high homogeneity induced pluripotent stem cell culture. This new 145 aa thermostable FGF-2 protein allows direct comparison of efficacy in systems using FGF-2 145 aa (Qk025) for chemically defined stem cell and organoid culture media, and cultured meat media development.
High purity 16 kDa bioactive FGF2-G3 145 aa protein, animal origin-free (AOF), carrier protein-free and with no His-tag.
QK053
Recombinant FGF2-G3 (FGF2-STAB®) protein is a thermostable engineered form of FGF-2 (bFGF). Qk053 is the 154 aa mature domain of FGF-2 (Qk027) with nine amino acid substitutions to enhance stability without impacting bioactivity developed by Dvorak et al. 2018. This increases the functional half-life of the protein from <10 h (wild-type) to >7 days (FGF2-G3).
Recombinant FGF2-G3 is used in B8 media (Kuo et al. 2019) for weekend free, high homogeneity induced pluripotent stem cell culture. FGF2-G3 also has applications in chemically defined stem cell and organoid culture media, and cultured meat media development.
High purity 17 kDa bioactive FGF2-G3 protein, animal origin-free (AOF), carrier protein-free and tag free.
QK067
Human recombinant activin E PLUS protein is an optimised biologically active truncation of the mature domain of human activin E protein. Activin E is a member of the transforming growth factor-beta (TGF-β) superfamily, a group of proteins that play key roles in regulating cell growth, differentiation, and inflammation. The tag-free form of activin E PLUS is of significant interest for drug discovery across various fields. An Avi-tag version, recombinant human activin E PLUS Avi-tag protein (Qk082), is also available upon request.
High purity 23.2 kDa dimer comprising truncated mature domain, animal origin-free (AOF) and carrier-protein free (CF).
QK050
Brain-derived neurotrophic factor (BDNF) is a member of neurotrophin family and plays a crucial role in neural development, maintenance, and function. It stimulates neurogenesis and is also a major regulator of synaptic plasticity and neuroprotection. It is used to maintain neurons and differentiate and mature human pluripotent stem cell-derived neural progenitors to cortical and motor neurons and cortical organoids.
Recombinant human BDNF protein has a molecular weight of 14 kDa. This protein is animal origin-free, carrier-free, tag-free, and non-glycosylated to ensure its purity with exceptional lot-to-lot consistency. Qk050 is suitable for the culture of reproducible and high-quality cortical and motor neurons and cortical organoids.
QK063
Ciliary Neurotrophic Factor (CNTF) is a member of the IL-6 family of cytokines. CNTF plays a crucial role in developing and maintaining the nervous system, in particular the optic nervous system. It promotes the maintenance, differentiation, and survival of various neurons, glial cells, and retinal cells.
CNTF has been used in vitro to initiate neural induction and differentiation. CNTF can be used to culture primary neurons and glial cells such as astrocytes and Schwann cells. It is also used to culture retinal cells and adipocytes.
Human CNTF has a molecular weight of 22.8 kDa. This protein is animal origin-free, carrier-free, tag-free, and non-glycosylated to ensure its purity with exceptional lot-to-lot consistency. Qk063 is suitable for the culture of reproducible and high-quality neurons and other relevant cells.
QK011
Human epidermal growth factor (EGF) protein is a potent EGF-family growth factor used in many human and mouse organoid and stem cell culture systems including intestinal and tumor organoid culture. EGF is also used in epithelial cell culture.
Qkine human EGF (Qk011) is a highly pure, animal origin-free, bioactive 6.3 kDa recombinant protein, making it ideal for use in chemically defined induced pluripotent stem cell (iPSC), embryonic stem cell (ESC) and organoid culture media.
QK071
Fibroblast Growth Factor 1 (FGF-1) can stimulate growth and differentiation of endothelial and epithelial cells and the development of organoids. FGF-1 can also be used for the maintenance of oligodendrocytes and astroglia as well as bone marrow-derived mesenchymal and hematopoietic stem cells.
Qkine human FGF-1 has a molecular weight of 15.9 kDa. This protein is animal origin-free, carrier-free and tag-free to ensure its purity with exceptional lot-to-lot consistency. Qk071 is suitable for the culture of reproducible mesenchymal, endothelial, haematopoietic, glial, and other relevant cells.
QK069
Fibroblast growth factor 18 (FGF-18), a member of the FGF family, characterized by its heparin-binding properties plays a significant role in regulating diverse biological processes such as embryonic development, skeletal and bone development, cartilage maintenance, angiogenesis and tissue repair.
In cell culture, FGF-18 is widely used to support cell culture maintenance and proliferation, promote chondrogenic and osteogenic differentiation of stem cells, stimulate angiogenesis, and enhance tissue regeneration.
FGF-18 is a high purity truncated protein with a molecular weight of 20.2 kDa. This protein is animal origin-free, carrier-free and tag-free to ensure its purity with exceptional lot-to-lot consistency. Qkine fibroblast growth factor 18 is suitable for the culture of reproducible and high-quality stem cells, primary cells and other relevant cells.
QK004
Human fibroblast growth factor 4 (FGF-4) protein is used for the proliferation and differentiation of embryonic and induced-pluripotent and tissue (mesenchymal) stem cells and promotes neural stem cell proliferation. Recombinant FGF-4 is an important component of cardiac, intestinal and other organoid culture media.
High purity and bioactivity 14 kDa, bioactive domain of human FGF-4, animal origin-free (AOF) and carrier-protein free.
QK059
Fibroblast growth factor 8a (FGF-8a) is a member of the FGF family and plays a key role in the regulation of embryogenesis, cellular proliferation, differentiation, and migration. FGF-8a is often used for the differentiation of induced pluripotent stem cells, embryonic stem cells, and neural stem cells.
FGF-8a is a spliced form of FGF-8, a heparin-binding protein that targets mammary and non-mammary cells expressing the FGF receptors. A 21.3 kDa highly pure, bioactive recombinant protein produced in an animal-free expression system. This protein is animal-origin free (AOF), carrier protein-free, tag-free, and non-glycosylated to ensure a pure homogenous protein with exceptional lot-to-lot consistency. Qk059 is suitable for enhanced reproducibility and physiologically relevant cultures.
QK057
Fibroblast growth factor 8b (FGF-8b) is a member of the FGF family involved in the regulation of embryogenesis, cellular proliferation, differentiation, and migration.
FGF-8b is commonly used for the differentiation of induced pluripotent stem cells into neural cell types and brain organoid cultures.
FGF-8b is a spliced form of FGF-8, a heparin-binding protein that targets mammary and non-mammary cells expressing the FGF receptors. A 22.5 kDa highly pure, bioactive recombinant protein produced in an animal origin-free expression system. This protein is carrier-free, tag-free and non-glycosylated to ensure a pure, homogenous protein with exceptional lot-to-lot consistency. Qk057 is suitable for enhanced reproducibility and physiologically relevant cultures.
QK039
Human FGF-9 protein is a member of the fibroblast growth factor (FGF) family, FGF-9 is involved in many developmental processes including neural and skeletal development. Recombinant FGF-9 is used frequently in the generation of kidney organoids.
Qkine recombinant human FGF-9 is a highly pure and bioactive 23 kDa protein, animal origin-free (AOF) and carrier-protein free (CF).
QK087
Fms-like tyrosine kinase 3 ligand (Flt-3 Ligand or Flt3L) is a cytokine that is involved in the regulation of hematopoiesis. It stimulates the survival, proliferation, and differentiation of various early myeloid and lymphoid progenitor cells. Flt3L is commonly used in the differentiation of hematopoietic stem cells into dendritic cells.
Human recombinant Flt3L has a molecular weight of 17.6 kDa. This protein is animal origin-free, carrier protein-free, tag-free, and non-glycosylated to ensure a homogenous population with exceptional lot-to-lot consistency. Flt3L is suitable for the culture of reproducible and high-quality myeloid progenitors and dendritic cells.
QK074
Granulocyte colony-stimulating factor (G-CSF) is a member of the hematopoietic growth factor family which plays a crucial role in the proliferation, differentiation, and maturation of committed progenitor cells to granulocytes, such as neutrophils.
Qkine G-CSF is a highly pure, bioactive glycoprotein produced in an animal origin-free expression system composed of 174 amino acids with a molecular weight of 18.6 kDa. Human and murine G-CSF are 73% identical at the amino acid level and show species cross-reactivity. Human G-CSF is carrier-free, tag-free, and non-glycosylated to ensure a pure and homogenous production of high-quality neutrophils and other relevant cell cultures with exceptional lot-to-lot consistency.
QK017
Human growth differentiation factor 15 (GDF-15) protein is a member of the TGFβ family and subject of intense interest as a marker of cellular stress and for its role in metabolism, cancer and pregnancy. Human GDF-15 also is functional in mouse studies.
Qkine GDF-15 is a 25 kDa disulfide-linked dimer composed of the mature domain of human GDF-15 protein. Our recombinant GDF-15 protein is exceptionally high purity, animal origin-free and extensively validated to ensure no trace contamination of related TGF-β family proteins from the mammalian culture systems.
QK070
Growth differentiation factor 5 (GDF-5) plays a crucial role during embryonic development and tissue homeostasis and is specifically involved in the development of the skeletal system. Recombinant human GDF-5 protein is commonly used for the differentiation and maintenance of induced pluripotent stem cells, embryonic stem cells, or bone marrow-derived mesenchymal stem cells into osteoblasts and chondrocytes.
Human GDF-5 is a dimer with a molecular weight of 27 kDa. This protein is animal origin-free, carrier protein-free, and tag- free with exceptional lot-to-lot consistency. GDF-5 is suitable for reproducible and high-quality chondrocytes and osteoblasts.
QK051
Glial cell line-derived neurotrophic factor (GDNF) is a member of neurotrophin family and GDNF family of ligands (GFL). GDNF plays a crucial role in the development, growth, and survival of neurons in particular midbrain dopaminergic neurons. GNDF is used to maintain neurons and cortical organoids and to differentiate dopaminergic neurons from human pluripotent stem cell-derived neural progenitors. GDNF also facilitates the differentiation of neural progenitors to astrocytes.
Recombinant human GDNF bioactive 30 kDa homodimer. This protein is animal origin-free (AOF), carrier protein-free, and tag-free to ensure its purity with exceptional lot-to-lot consistency.
QK076
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a hematopoietic growth factor involved in the differentiation and activation of monocytes such as macrophages and dendritic cells, and granulocytes such as neutrophils, eosinophils, and basophils.
GM-CSF is commonly used in cell culture to stimulate the differentiation and maturation of human-induced pluripotent stem cells or peripheral blood monocytes to myeloid cells.
Qkine human GM-CSF is composed of 144 amino acids with a molecular weight of 14.6 kDa. This protein is animal origin-free, carrier protein-free, tag-free, and non-glycosylated to ensure a homogenous population with exceptional lot-to-lot consistency. Qk076 is suitable for reproducible and high-quality neutrophils and other relevant cell cultures.
QK117
IFN-gamma (interferon-gamma) is a cytokine crucial for immune responses, produced by T cells and natural killer cells. It activates macrophages, enhances antigen presentation, and plays a key role in inflammation.
Qkine has optimised the IFN-gamma manufacture process to produce a highly bioactive protein with excellent lot-to-lot consistency for enhanced experimental reproducibility. Qkine IFN-gamma is a highly pure 16.8 kDa protein, animal origin-free (AOF) and carrier-protein-free (CF).
QK047
Human insulin-like growth factor 1 (IGF-1) protein is used in the maintenance of human pluripotent stem cells (IPSC) and is necessary for cell growth in the absence of insulin. IGF-1 promotes the proliferation of many cell types, including embryonic stem cells (ESC) and mesenchymal stem cells (MSC).
Qkine IGF-1 is a highly pure and bioactive 8 kDa protein, Qkine growth factors are animal origin-free (AOF) and carrier-protein free (CF) for consistent stem cell culture.
QK118
Insulin-like Growth Factor 2 (IGF-2) is a growth factor that promotes cell proliferation, differentiation, and survival, particularly during fetal development. IGF-2 also regulates tissue growth and muscle regeneration.
Qkine has optimised the IGF-2 manufacture process to produce a highly bioactive protein with excellent lot-to-lot consistency for enhanced experimental reproducibility.
Qkine human IGF-2 is a highly pure 7.5 kDa monomer, animal origin-free (AOF) and carrier-protein-free (CF).
QK101
Recombinant human interleukin-1 beta (IL-1β) is a pro-inflammatory cytokine and a potent mediator of inflammation and immune responses. It promotes the activation and recruitment of immune cells to sites of infection or injury, such as antigen presenting cells, macrophages, and T lymphocytes. In cell culture, IL-1β is used to stimulate and activate immune cells (macrophages and T cells), maintain hematopoietic progenitor cells, and modulate the differentiation of mesenchymal stem cells.
Human IL-1β is a monomer of a molecular weight of 17.3 kDa. This protein is animal origin-free, carrier protein-free, and tag-free to ensure a homogenous population with exceptional lot-to-lot consistency. IL-1β is suitable for reproducible, high-quality hematopoietic stem cells and more specific lineages.
QK089
Interleukin-2 (IL-2) is a vital cytokine that regulates immune responses by promoting the growth and activation of T-cells, enhancing immune function, and maintaining immune tolerance for immune activation and suppression.
Qkine has optimised the IL-2 manufacture process to produce a highly bioactive protein with excellent lot-to-lot consistency for enhanced experimental reproducibility. Qkine IL-2 is a highly pure 15.5 kDa monomer, animal origin-free (AOF) and carrier-protein-free (CF).
QK090
Recombinant Human Interleukin 3 (IL-3) is a pleiotropic cytokine which plays a crucial role in hematopoiesis and acts as a multi-lineage growth factor. As a hematopoietic growth factor, it promotes the proliferation, differentiation, and survival of various hematopoietic stem cells towards myeloid progenitors. IL-3 is commonly used in cell culture to stimulate the differentiation and maturation of human induced pluripotent stem cells towards mast cells, basophils, neutrophils, eosinophils, monocytes, and megakaryocytes.
Human IL-3 comprises 140 amino acids with a molecular weight of 15.2 kDa. This protein is animal origin-free, carrier protein-free, and tag-free to ensure a homogenous population with exceptional lot-to-lot consistency. IL-3 is suitable for reproducible and high-quality myeloid progenitors and more specific lineages.
QK092
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine that is secreted primarily by mast cells, T-cells, eosinophils, and basophils. IL-4 plays a crucial role in hematopoiesis, the regulation of antibody production, the stimulation of activated B cell and T cell proliferation, and the differentiation of B cells into plasma cells
Human IL-4 has a molecular weight of 15.1 kDa. This protein is animal origin-free, carrier-free and tag-free to ensure its purity with exceptional lot-to-lot consistency. Qk092 is suitable for the culture of reproducible and high-quality stem cells and other relevant cells.
QK093
Interleukin-6 (IL-6) is a multifunctional cytokine that regulates immune responses and inflammation. It is produced by various cells, including immune cells such as T cells and macrophages, as well as non-immune cells like fibroblasts and endothelial cells.
Human IL-6 has a molecular weight of 20.9 kDa. This protein is animal origin-free, carrier-free and tag-free to ensure its purity with exceptional lot-to-lot consistency. IL-6 is suitable for the culture of reproducible and high-quality hematopoietic stem cells and other relevant cells.
QK095
Interleukin-7 (IL-7) is a vital cytokine essential for immune system regulation, particularly in the development and maintenance of T cells, playing a crucial role in both adaptive and innate immune responses. Recombinant human IL-7 stimulates the development of lymphoid progenitor cells.
Qkine has optimised the IL-7 manufacture process to produce a highly bioactive protein with excellent lot-to-lot consistency for enhanced experimental reproducibility.
IL-7 is a highly pure 17.5 kDa monomer, animal origin-free (AOF) and carrier-protein-free (CF).
QK046
Human KGF protein (FGF-7) is a member of the FGF family. KGF protein is used in the culture of lung, breast, liver, and bladder organoids.
Qkine human FGF-7 / KGF is a highly pure and bioactive 19 kDa protein, animal-origin free (AOF) and carrier-protein free (CF).
QK075
Macrophage colony-stimulating factor (M-CSF) is a cytokine that regulates the survival, proliferation, differentiation, and functional activation of monocytes, such as macrophages and dendritic cells.
M-CSF has been used in vitro for the differentiation of human induced pluripotent stem cell (iPSC)-derived macrophages as well as the maintenance and development of monocytes in hematopoietic stem cell culture. M-CSF has also been used to influence macrophage polarization towards an anti-inflammatory or M2 macrophage phenotype.
Qkine M-CSF is a highly bioactive, animal origin-free (AOF), carrier protein-free 36.8 kDa recombinant protein with exceptional lot-to-lot consistency.
QK034
Human noggin is a bone morphogenetic protein (BMP) antagonist which regulates cell differentiation and growth. Noggin is used in the culture of intestinal, pancreatic, lung and tumor-derived organoids and the maintenance of undifferentiated embryonic stem cells (ESC) and for stem cell differentiation into neural and microglial lineages.
Qkine human noggin (Qk034) is a highly pure, animal origin-free, bioactive 46 kDa dimer for reproducible results in organoid culture.
QK058
Neurotrophin 3 (NT-3) protein is part of the neurotrophin family and plays a crucial role in embryonic development and the maintenance and neuroprotection of the adult nervous system. NT-3 protein is used in cell culture to promote the differentiation and survival of specific neural subpopulations in both the central nervous system and peripheral nervous system such as sensory neurons, cortical neurons, and oligodendrocytes. It is also involved in the maintenance of endothelial cells and myocardial cells.
Qkine NT-3 protein is a non-covalently linked homodimer with a molecular weight of 27.3 kDa, animal origin-free (AOF), carrier-free, tag-free, and non-glycosylated to ensure its purity with exceptional lot-to-lot consistency. It is suitable for the culture of reproducible and high-quality cortical neurons and oligodendrocytes.
QK049
Human OSM protein (oncostatin M) is used in the differentiation of human pluripotent stem cells into hepatocyte-like cells. OSM also plays roles in osteogenesis and neurogenesis, and is an important regulator of the hematopoietic stem cell niche in the bone marrow.
Qkine human oncostatin M protein is high purity and animal origin-free (AOF) and carrier-protein free for reproducible results.
QK043
Human PDGF-AA (platelet derived growth factor AA) protein is a homodimer of PDGF-A peptide chains. PDGF-AA is commonly used to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into oligodendrocyte precursor cells.
Qkine human PDGF-AA is a high purity 29 kDa disulfide–linked bioactive dimeric protein, animal origin-free (AOF) and carrier protein-free.
QK044
Human PDGF-BB (platelet derived growth factor BB) protein is a homodimer of PDGF-B peptide chains. Its is a potent mitogen expressed by platelets which stimulates proliferation and angiogenesis and is involved in tissue repair. PDGF-BB increases stem cell proliferation and marker expression and can be used in osteogenic and neural stem cell differentiation.
Qkine recombinant human PDGF-BB is a highly pure 25 kDa disulfide–linked bioactive dimeric protein, animal origin-free (AOF), tag free and carrier protein-free.
QK031
Recombinant human R-spondin 1 LR5 protein is engineered to act as a high affinity ligand for the LGR5 receptor. In epithelial tissues LGR5 marks the stem cell population. This engineered protein, R-spondin 1 LR5, activates Wnt signalling only in the LGR5+ stem cell population.
R-spondin 1 LR5 has been tested in intestinal organoid culture and supports organoid survival and growth. As LGR5 specifically marks stem cells and is not found on transit amplifying cells, the lower crypt multiplicity seen in organoid cultures with R-spondin 1 LR5 (in comparison to wild-type R-spondin 1, Qk006) supports the notion that this engineered form is acting specifically on stem cells. This specialized form of R-spondin 1 was developed in Marc de la Roche’s lab (University of Cambridge).
QK006
R-spondin 1 protein (RSPO1) is the prototypic member of the R-spondin family and is used to potentiate Wnt signaling in many organoid culture systems including intestinal and tumor (cancer) organoid culture. R-spondin 1 is also required for hematopoietic stem cell specification and cancer cell migration and survival.
Recombinant human R-spondin 1 protein (RSPO1) protein is the bioactive domain of human R-spondin 1 comprising the two cysteine-rich furin-like domains of R-spondin 1. Those are necessary and sufficient for Wnt signaling potentiation and are the essential domains for activity in stem cell and organoid culture.
This protein has a molecular weight of 13 kDa and is animal origin-free (AOF), carrier protein-free, and tag-free to ensure its purity with exceptional lot-to-lot consistency. Qk006 is suitable to replace R-spondin conditioned media for improved reproducibility in chemically defined organoid culture media.
QK032
Human R-spondin 3 protein potentiates Wnt signalling in and has been shown to function in crypt regeneration in the intestine and control stem cell and progenitor cell behaviour during kidney development. R-spondin 3 is used alongside R-spondin 1 in intestinal organoid culture systems.
Qk032 is a 17 kDa highly pure, bioactive domain of human R-spondin 3 comprising the two cysteine-rich furin-like domains, which are necessary and sufficient for Wnt signalling potentiation and are the essential domains for activity in stem cell and organoid culture. Animal origin-free and carrier-protein free.
QK078
Stem Cell Factor (SCF) is a critical factor in the maintenance and expansion of hematopoietic stem cells (HSCs) in the bone marrow microenvironment. Key myeloid progenitor differentiation factor for a variety of myeloid cells such as megakaryocytes, basophils, neutrophils, and monocytes, Stem Cell Factor is also a primary growth and activation factor for mast cells and eosinophils.
Recombinant SCF is a highly pure 18.5 kDa monomer, animal origin-free (AOF) and carrier-protein-free (CF). Qkine has optimised the SCF manufacture process to produce a highly bioactive protein with excellent lot-to-lot consistency for enhanced experimental reproducibility.
Recombinant human Shh protein Brand: Qkine
QK055
Sonic hedgehog protein (Shh) is a member of the Hedgehog family with an essential role in embryonic development, tissue regeneration, and tumorigenesis. Shh induces the cell fate and patterning of neural progenitors in ventral domains at various levels in the forebrain, midbrain, hindbrain, and spinal cord. It has many applications in the neural stem cell field where it plays a significant role in differentiating human-induced pluripotent stem cells (iPSC) towards motor neurons and interneurons. Also, it induces the patterning of organoids and embryos in culture.
Recombinant Shh protein has a molecular weight of 19.8 kDa. This protein is animal origin-free (AOF), carrier protein-free, and tag-free to ensure its purity with exceptional lot-to-lot consistency. Sonic hedgehog protein is suitable for the culture of reproducible and high-quality neurons and organoids.
QK072
Transforming Growth Factor beta 2 (TGF-β2), part of the TGF-β superfamily, regulates a wide array of cellular processes, including proliferation, differentiation, wound healing, apoptosis, metabolism, embryogenesis, and tissue repair. It is an essential growth factor in many embryonic and induced pluripotent stem cell culture media.
TGF-β2 is a high purity protein with a molecular weight of 25.4 kDa. This protein is carrier-free and tag-free to ensure its purity with exceptional lot-to-lot consistency. Qkine TGF-β2 is suitable for the culture of reproducible and high-quality stem cells and other relevant cells.
QK054
Human transforming growth factor beta 3 (TGF-β3) protein is a member of the TGF-β family, a family involved in regulating cell survival, proliferation and differentiation. TGF-β3 is used in human pluripotent stem cell maintenance media.
Qkine TGF-β3 is a highly pure and bioactive 25.4 kDa protein dimer. Qkine growth factors are animal-origin free (AOF) and carrier-protein free (CF) for reproducible results.
QK083
Tumor necrosis factor-alpha (TNF-α) is a cytokine involved in cell signaling, primarily produced by immune cells like macrophages. It plays a central role in inflammation, immunity, and regulating cell survival and death.
Human TNF-α has a molecular weight of 17.3 kDa, animal origin-free, carrier-free and protein tag-free to ensure its purity with exceptional lot-to-lot consistency. Qk083 TNF-alpha is suitable for the culture of reproducible and high-quality hematopoietic stem cells and other relevant cells.
QK048
Recombinant human vascular endothelial growth factor 165 (VEGF165/ VEGF-165/ VEGF165) protein is widely used in culturing primary endothelial cells, such as human umbilical vein endothelial cells (HUVEC).
VEGF 165 is commonly used with human-induced pluripotent stem cells or embryonic stem cells-derived endothelial cells for developing human vascular tissue models. It has many applications including its use in neural research involving oligodendrocyte precursor cells, Schwann cells, astrocytes, and microglia. It plays a role in bone formation, regulates mesenchymal stem cell differentiation, and serves as a survival factor for chondrocytes, hematopoietic stem cells, and tumor cells.
Qkine recombinant human VEGF 165 protein is a highly pure and potent 38 kDa homodimer, disulfide-linked consisting of two 165 amino acid polypeptide chains. It is an animal origin-free (AOF) and carrier protein-free (CF).
QK120
Vitronectin protein is widely used in stem cell culture. It provides a defined environment that supports the maintenance of pluripotency and is suitable for feeder-free culture, expansion, differentiation, and reprogramming of stem cells.
Qkine vitronectin is a high-purity animal origin-free recombinant protein with a molecular weight of 47.8 kDa. It is carrier-free and protein tag-free, ensuring exceptional lot-to-lot consistency and compatibility with translational studies and iPSC-based model production for screening. Qkine recombinant vitronectin protein is ultra high-purity with exceptionally low residual endotoxins making it suitable for the reproducible culture of stem cells, primary cells, organoids, and use in sensitive neural differentiations.
QK015
Human gremlin 1 protein is a BMP-inhibitor present in the natural intestinal niche and provides an alternative to noggin for optimisation of intestinal organoid culture and iPSC differentiation.
Qkine gremlin 1 has been optimized by our experts to be an exceptionally high-purity 18 kDa highly bioactive dimeric protein, animal origin-free (AOF) and carrier protein free.
QK041
Human insulin-like growth factor 1 long arginine 3 (IGF-1 LR3) protein is a synthetic analog of IGF-1. The substitutions include an arginine substitution and an N-terminal protein extension. Consequently, IGF-1 LR3 has improved biological potency and extended half-life. IGF-1 LR3 can be used for the consistent, reproducible maintenance of human pluripotent stem cells.
Qkine IGF-1 LR3 is a highly pure and bioactive 9 kDa protein monomer, animal origin-free (AOF) and carrier-protein free (CF).
QK045
Human NRG-1 protein (neuregulin 1) is frequently used in the maintenance of human pluripotent stem cells. In addition to its widespread use in stem cell culture media, NRG-1 (also known as Heregulin-β1 , HRG-1) has essential roles in vivo including in nervous system, cardiac, and mammary gland development; cancer biology and neurological disorders.
7.5 kDa highly pure, bioactive domain of human NRG-1, comprised of the β isoform of the EGF-like domain of NRG-1 (HRG1-B1). This NRG-1 protein monomer is animal origin-free (AOF) and carrier-protein free (CF).
QK010
Transforming growth factor-beta 1 (TGF-β1) is a pleiotropic cytokine that regulates various cellular processes, including cell proliferation, growth, differentiation, motility, and apoptosis. It is an essential growth factor in many embryonic and induced pluripotent stem cell maintenance media, including the commonly used E8, StemPro, and mTeSR media. TGF-β1 also promotes the differentiation of various cell types such as fibroblasts, epithelial cells, and immune cells.
Human recombinant TGF-β1 PLUS™ protein is the first entirely animal origin-free recombinant human TGF-β1 protein for highly reproducible results and compatible with chemically-defined stem cell media.
TGF-β1 PLUS™ is a high purity 24 kDa dimer comprising optimized mature domain of TGF-β1 protein. Our TGF-β1 PLUS™ protein has been extensively tested for maintenance of iPSC pluripotency by the specialist stem cell biotechnology company, Stemnovate, Cambridge, UK.
QK035
Follistatin-resistant activin A (FRACTA) protein has been engineered to prevent binding to the natural inhibitor, follistatin. In vivo activin A activity is regulated by follistatin, a high-affinity inhibitor; follistatin accumulates in stem cell culture, where it inhibits activin A.
Qk035 follistatin-resistant activin A (FRACTA) has equivalent bioactivity to wild-type activin A (Qk001) but does not bind follistatin so is resistant to feedback inhibition. High purity 26 kDa dimer comprising engineered mature domain of activin A protein, animal origin-free (AOF) and carrier-protein free (CF). This specialised activin A has been developed in Marko Hyvönen’s group in the University of Cambridge.
QK007
Human recombinant BMP-2 protein (bone morphogenetic protein 2) protein is member of the TGFβ family and a key regulator of embryogenesis and potent differentiation factor of embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC) towards endoderm fates. BMP-2 plays roles in the differentiation of mesenchymal cells to adipocytes, epithelial cancer EMT, chondrogenesis and regulation of neuronal and glial cell development.
26 kDa disulfide–linked bioactive highly pure dimer comprised of the mature domain of human BMP-2 protein, animal origin-free (AOF) and carrier protein-free.
QK003
Human/rat/porcine/bovine fibroblast growth factor 10 (FGF-10) protein promotes lung organoid formation and induces branching morphology. FGF-10 protein is used widely in organoid culture, embryonic stem cell (ESC) and induced-pluripotent stem cell (iPSC) differentiation, and for the study of epithelial to mesenchymal transition (EMT) and tumor metastasis.
Qkine Qk003 is a high purity and bioactivity 17 kDa bioactive domain of human FGF-10, animal origin-free (AOF) and carrier-protein free (CF).
