Induced pluripotent stem cells (iPSCs) are rapidly advancing regenerative medicine and cell therapy because they can differentiate into multiple human cell types. However, immune rejection remains a major barrier to developing scalable allogeneic cell therapies. In a recent webinar, Dr. Luke Ren from REPROCELL presented how the company is using StemEdit, its advanced gene editing…
What Are Off-target Effects in Gene Editing? Off-target effects in gene editing refer to unintended genetic modifications occurring at genomic loci other than the intended target site. In CRISPR-based gene editing systems, these arise because the guide RNA (gRNA), which directs the system to the target DNA sequence, can also bind to DNA sequences with…
What Is Custom DNA Oligonucleotide Synthesis? Custom DNA oligonucleotide synthesis is the chemical process of building short, single-stranded DNA sequences—called oligonucleotides or “oligos”—in a controlled, laboratory setting. These synthetic DNA molecules are indispensable tools in modern molecular biology, genomics, diagnostics, and therapeutic development. Whether you are designing primers for PCR amplification, probes for next-generation sequencing…
Stem cell research has entered a decisive translational era. Breakthroughs in induced pluripotent stem cell (iPSC) technology and precision gene editing are rapidly converting experimental concepts into clinically validated therapies. What were once proof-of-concept studies are now progressing through late-stage clinical trials and, in some cases, delivering first-in-human successes. These advances are unlocking new therapeutic frontiers…
Induced pluripotent stem cells (iPSCs) are among the most transformative innovations in modern regenerative medicine. The concept itself seems almost futuristic: by taking a small sample of somatic cells, such as skin fibroblasts, and reprogramming them back into a pluripotent state. These reprogrammed cells can give rise to diverse cell types, opening new possibilities for personalized medicine, disease modelling, and tissue regeneration.
Classic epigenetic modifications include DNA methylation and histone modifications as well as more broadly chromatin accessibility and non-coding RNAs. Epigenetic mechanisms enable heritable changes in gene expression that occur without altering the underlying DNA sequence. The various epigenetic layers act to control DNA accessibility and chromatin structure, which defines transcription factor binding and downstream gene…
1. Ethical and Regulatory Compliance The challenge: Clinical and commercial applications require strict ethical oversight and compliance with global regulatory standards. Our solution: REPROCELL follows IRB-approved informed consent protocols, and the clinical iPSC generation is compliant with all major regulatory standards (FDA, EMA, PMDA). We ensure: Full donor de-identification Ethical donor recruitment Global consent for clinical and…
The Power of mRNA Reprogramming The emergence of mRNA reprogramming offers a precise, efficient, and xeno-free approach to generating induced pluripotent stem cells (iPSCs). These versatile cells hold immense potential for personalized medicine, disease modelling, and drug discovery. REPROCELL has led the way in mRNA reprogramming, in 2010 becoming the first company to commercialize mRNA-based…
Induced Pluripotent Stem Cells (iPSCs) are revolutionizing regenerative medicine. They play a pivotal role in disease modeling and modern cell therapy development. One of the challenges with cell-based models is scale: How do we get sufficient cells at an affordable cost? Suspension cultures using bioreactors offer a solution.Bioreactors are available from several vendors in scales…
Custom DNA Oligonucleotide Synthesis Services: Precision Tools for Molecular Success In modern molecular biology, custom DNA oligonucleotides (or “oligos”) are indispensable tools used in diagnostics, gene editing, synthetic biology, and research. As the demand for personalized, high-quality DNA sequences grows, so does the need for reliable custom oligo synthesis services tailored to specific experimental needs.…